Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl-Coenzyme A:Acetate/Butyrate:Coenzyme A-Transferase (EC 2.8.3.9) and in Other Solvent Pathway Enzymes.

@article{Clark1989IsolationAC,
  title={Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl-Coenzyme A:Acetate/Butyrate:Coenzyme A-Transferase (EC 2.8.3.9) and in Other Solvent Pathway Enzymes.},
  author={Steven W. Clark and George N. Bennett and Frederick B. Rudolph},
  journal={Applied and environmental microbiology},
  year={1989},
  volume={55 4},
  pages={970-6}
}
Mutants of Clostridium acetobutylicum ATCC 824 exhibiting resistance to 2-bromobutyrate or rifampin were isolated after nitrosoguanidine treatment. Mutants were screened for solvent production by using an automated alcohol test system. Isolates were analyzed for levels of butanol, ethanol, acetone, butyrate, acetate, and acetoin in stationary-phase batch cultures. The specific activities of NADH- and NADPH-dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of… CONTINUE READING

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Isolates were analyzed for levels of butanol , ethanol , acetone , butyrate , acetate , and acetoin in stationary - phase batch cultures .
Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl - Coenzyme A : Acetate / Butyrate : Coenzyme A - Transferase ( EC 2.8.3.9 ) and in Other Solvent Pathway Enzymes .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl - Coenzyme A : Acetate / Butyrate : Coenzyme A - Transferase ( EC 2.8.3.9 ) and in Other Solvent Pathway Enzymes .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
Isolates were analyzed for levels of butanol , ethanol , acetone , butyrate , acetate , and acetoin in stationary - phase batch cultures .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl - Coenzyme A : Acetate / Butyrate : Coenzyme A - Transferase ( EC 2.8.3.9 ) and in Other Solvent Pathway Enzymes .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl - Coenzyme A : Acetate / Butyrate : Coenzyme A - Transferase ( EC 2.8.3.9 ) and in Other Solvent Pathway Enzymes .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl - Coenzyme A : Acetate / Butyrate : Coenzyme A - Transferase ( EC 2.8.3.9 ) and in Other Solvent Pathway Enzymes .
Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl - Coenzyme A : Acetate / Butyrate : Coenzyme A - Transferase ( EC 2.8.3.9 ) and in Other Solvent Pathway Enzymes .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
The specific activities of NADH- and NADPH - dependent butanol dehydrogenase and butyraldehyde dehydrogenase as well as those of acetoacetyl - coenzyme A : acetate / butyrate : coenzyme A - transferase ( butyrate - acetoacetate coenzyme A - transferase [ EC 2.8.3.9 ] ) ( CoA - transferase ) , butyrate kinase , and phosphotransbutyrylase were measured at the onset of stationary phase .
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