Isolation and Characterization of Human Clonogenic Osteoblast Progenitors Immunoselected from Fetal Bone Marrow Stroma Using STRO‐1 Monoclonal Antibody

@article{Oyajobi1999IsolationAC,
  title={Isolation and Characterization of Human Clonogenic Osteoblast Progenitors Immunoselected from Fetal Bone Marrow Stroma Using STRO‐1 Monoclonal Antibody},
  author={B. Oyajobi and A. Lomri and M. Hott and P. Marie},
  journal={Journal of Bone and Mineral Research},
  year={1999},
  volume={14}
}
Osteoprogenitor cells present in human fetal bone marrow (BM) stroma have not been characterized. We used density gradient centrifugation, aggregation on binding lectin, and enrichment by magnetic activated cell sorting with STRO‐1 antibody to isolate STRO‐1+ cells from nonadherent human fetal BM stromal cells. Immunoselected STRO‐1+ cells were immortalized using SV‐40 large T antigen and a clone, F/STRO‐1+ A, with weak alkaline phosphatase (ALP) activity was selected. The cloned cells… Expand
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