Isolation and Characterization of Hemolymph Clotting Factors in Drosophila melanogaster by a Pullout Method

@article{Scherfer2004IsolationAC,
  title={Isolation and Characterization of Hemolymph Clotting Factors in Drosophila melanogaster by a Pullout Method},
  author={C. Scherfer and C. Karlsson and O. Loseva and G. Bidla and A. Goto and J. Havemann and M. Dushay and U. Theopold},
  journal={Current Biology},
  year={2004},
  volume={14},
  pages={625-629}
}
Clotting is critical in limiting loss of hemolymph and initiating wound healing in insects as well as in vertebrates. Clotting is also an important immune defense, quickly forming a secondary barrier to infection, thereby immobilizing, and possibly killing bacteria directly. Here, we describe methods to assess clotting and to extract the clot from Drosophila larval hemolymph by using aggregation of paramagnetic beads. The validity of the assay was demonstrated by characterization of mutants. We… Expand
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TLDR
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Role and Importance of Phenoloxidase in Insect Hemostasis
TLDR
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TLDR
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