Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts ( Vigna radiata L )

Abstract

The diamine oxidase enzyme (DAO) can be isolated from mung bean sprouts (Vigna radiata L) through the process of extraction in phosphate buffer. Concentration and fractionation were performed by the addition of ammonium sulphate, (NH4)2SO4 saturation in stages and cold centrifuged at 10000 rpm. Enzyme activity of DAO crude and DAO fractionation results were… (More)

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