Isolation Conditions for High Yields of Protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae)

  title={Isolation Conditions for High Yields of Protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae)},
  author={Declan Butler and Kjetill {\O}stgaard and Catherine Boyen and Leonard V. Evans and Arne Johan Jensen and Bernard Kloareg},
  journal={Journal of Experimental Botany},
Isolement de protoplastes de Laminaria en utilisant des melanges d'enzymes: cellulases commercialisees, mannuronate lyases hautement purifiees, guluronate lyases isolees de bacteries 
Efficient Isolation and Culture of Viable Protoplasts from Laminaria longissima Miyabe (Phaeophyceae)
This method is an easy and stable way to produce and culture protoplasts from Laminariales plants and was more suitable for survival and cell division than low temperatures.
Protoplasts of Laminaria digitata and Laminaria saccharina (Phaeophyta) — Cultivation and Biosynthesis of Alginate
The composition of alginates produced from regenerating protoplasts have been determined by NMR and by an enzymatic method and are found to be markedly influenced by the medium composition.
Isolation of protoplasts from Fucus serratus and F. vesiculosus(Fucales, Phaeophyceae): factors affecting protoplast yield
Protoplasts were isolated enzymatically from meristematic tissues of the brown algae, Fucus serratus, using a combination of 2% cellulase R-10 Onozuka, 0.5% macerozyme and 1% crude extract of gland
Factors affecting protoplast yield of the carrageenophyte Solieria filiformis (Gigartinales, Rhodophyta)
SummaryThe effect of age, pH of the culture medium, pre-treatment of tissues, enzymes sources and enzymatic adaptability of phycophages fed with a monospecific diet were analyzed on the protoplast
Protoplast isolation from Ulva rigida (Chlorophyta)
High numbers of protoplasts were isolated from wild and cultivated thalli of Ulva rigida and protoplasts were photosynthetically active, and 70–90% of the protoplast were estimated to be viable.
Protoplast production in Chondrus crispus gametophytes (gigartinales, rhodophyta)
Protoplasts were isolated from female gametophytes of Chondrus crispus (Stackh.) using commercial cellulase and various carrageenases prepared from marine bacteria, and most protoplasts were viable and regenerated a cell wall and divided.
Preparation of alginate lyases from marine molluscs for protoplast isolation in brown algae
Alginate lyases from both the sea hare Aplysia depilans and the abalone Haliotis tuberculata preferentially degraded polymannuronate sequences and did not retain cellulase, fucanase or proteolytic activities.
Mannuronan C-5 epimerase activity in protoplasts of Laminaria digitata
After cellular disruption by osmotic shock and centrifugation, most of the epimerase activity resided in the pellet fraction, which may indicate that the enzyme is membrane associated.
Chemical Composition of Protoplasts of Laminaria digitata (Phaeophyceae)
High numbers of protoplasts were isolated from Laminana digitata and seasonal variation in protein, total carbohydrate and mannitol content was observed in accordance with the variation found in the corresponding plant tissue.