Isolation, structure and antibacterial activity of pleosporone from a pleosporalean ascomycete discovered by using antisense strategy.

@article{Zhang2009IsolationSA,
  title={Isolation, structure and antibacterial activity of pleosporone from a pleosporalean ascomycete discovered by using antisense strategy.},
  author={Chaowei Zhang and John G. Ondeyka and Deborah L. Zink and Ángela Basilio and Francisca Vicente and Javier Collado and Gonzalo Platas and Joann Huber and Karen Dorso and Mary R. Motyl and Kevin M. Byrne and Sheo B. Singh},
  journal={Bioorganic \& medicinal chemistry},
  year={2009},
  volume={17 6},
  pages={
          2162-6
        }
}

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References

SHOWING 1-10 OF 20 REFERENCES
Isolation, structure, and absolute stereochemistry of platensimycin, a broad spectrum antibiotic discovered using an antisense differential sensitivity strategy.
TLDR
It was determined that potential reactivity of the enone moiety does not play a key role in the biological activity of platensimycin and cyclohexenone ring conformation renders for the stronger binding interaction with the enzyme.
Coniothyrione, a chlorocyclopentandienylbenzopyrone as a bacterial protein synthesis inhibitor discovered by antisense technology.
TLDR
The antisense-rpsD gene-sensitized two-plate assay led to the discovery of a novel chlorinated cyclopentandienylbenzopyrone antibiotic, coniothyrione, C14H9ClO6, isolated from Coniothyrium cerealis MF7209.
Platensimycin is a selective FabF inhibitor with potent antibiotic properties
TLDR
Platensimycin demonstrates strong, broad-spectrum Gram-positive antibacterial activity by selectively inhibiting cellular lipid biosynthesis through the selective targeting of β-ketoacyl-(acyl-carrier-protein (ACP) in the synthetic pathway of fatty acids.
Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties
TLDR
Platencin shows potent in vivo efficacy without any observed toxicity, emphasizing the fact that more antibiotics with novel structures and new modes of action can be discovered by using this antisense differential sensitivity whole-cell screening paradigm.
Discovery of FabH/FabF Inhibitors from Natural Products
TLDR
The mechanism of decreased target protein production by expression of antisense RNA was investigated using Northern blotting and revealed that the antisenseRNA acts posttranscriptionally by targeting mRNA, leading to 5′ mRNA degradation.
Discovery of lucensimycins A and B from Streptomyces lucensis MA7349 using an antisense strategy.
TLDR
This work describes, using an antisense-sensitized rpsD Streptomyces aureus strain, the discovery and activity of lucensimycins A and B, a potential target for the discovery of antibacterial agents.
Determination of Selectivity and Efficacy of Fatty Acid Synthesis Inhibitors*
TLDR
A high throughput assay that identifies inhibitors simultaneously against multiple targets within the FASII pathway of most bacterial pathogens and isolated an inhibitor, bischloroanthrabenzoxocinone (BABX) with a new structure was developed, which will aid in future drug discovery.
Antibacterial Agents That Inhibit Lipid A Biosynthesis
TLDR
Synthetic antibacterials were identified that inhibit the second enzyme (a unique deacetylase) of lipid A biosynthesis that constitutes the outer monolayer of the outer membrane of Gram-negative bacteria and is essential for bacterial growth.
A genome‐wide strategy for the identification of essential genes in Staphylococcus aureus
TLDR
A cell‐based, drug‐screening assay is described, wherein expression of an antisense RNA confers specific sensitivity to compounds targeting that gene product, thereby greatly facilitating the search for new antibiotics.
The rpsD gene, encoding ribosomal protein S4, is autogenously regulated in Bacillus subtilis
TLDR
The B. subtilis rpsD gene, encoding ribosomal protein S4, was found to be subject to autogenous control and exhibits little sequence homology to the E. coli alpha operon leader but may be able to form a pseudoknotlike structure similar to that found in E. Escherichia coli.
...
1
2
...