Isolation, purification and characterization of intact and pepsin-derived fragments of laminin from human placenta.

Abstract

Human laminin, in intact form and as pepsin cleaved fragments, was isolated and purified from placenta. The intact laminin was extracted by 1-M NaCl at neutral pH in the presence of 10-mM EDTA and 3% Triton X-100. This recovered material was purified by DEAE-cellulose and agarose gel chromatography. The laminin fragments P1, P2 and P3 were prepared by… (More)

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