Isolation, purification, and characterization of staphylocoagulase, a blood coagulating protein from Staphylococcus sp. MBBJP S43.

@article{Bharadwaz2017IsolationPA,
  title={Isolation, purification, and characterization of staphylocoagulase, a blood coagulating protein from Staphylococcus sp. MBBJP S43.},
  author={Moonmee Bharadwaz and Prasenjit Manna and Dhrubajyoti D. Das and Niren Kumar Dutta and Jatin Kalita and Bala Gopalan Unni and Hari Prasanna Deka Boruah},
  journal={International journal of biological macromolecules},
  year={2017},
  volume={102},
  pages={
          1312-1321
        }
}
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TLDR
The protein isolated from the strain showed coagulating property as quick as 30 seconds, and theoretical 3D structure of the protein depicts the presence of the one cavity having highest volume and surface volume among five present which is the binding site for prothrombin.
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The efficiency of the tube coagulase test can be markedly improved by sequel testing of the isolates with Mannitol salt agar, DNase and Tube coagULase.
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TLDR
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TLDR
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TLDR
It is reported here that Coa and vWbp together are required for the formation of abscesses, suggesting that coagulases function as protective antigens for a staphylococcal vaccine.
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TLDR
The protein showed maximum hepatoprotective activity when administered at a dose of 2 mg/kg body weight for five days after CCl4 administration and Histopathological studies also supported the hepatoprotsective nature of the protein.
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