Isolation, purification, and characterization of mouse placental lactogen.

  title={Isolation, purification, and characterization of mouse placental lactogen.},
  author={Peter Colosi and G A Marr and J. Gonzalez Lopez and Luis S Haro and L M Ogren and Frank J Talamantes},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={79 3},
Mouse placental lactogen was purified 1840-fold from BALB/c placentae from days 14-18 of gestation with an overall yield of 29%. The purification procedure included alkaline homogenization and extraction, ammonium sulfate precipitation, hydrophobic interaction chromatography on Phenyl-Sepharose, ion-exchange chromatography on CM- and DEAE-cellulose, and gel exclusion chromatography on Sephadex G-100. On 10% alkaline polyacrylamide gels, mouse placental lactogen had an Rf of 0.19… CONTINUE READING

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