Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae.

@article{Martins2011IsolationPC,
  title={Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae.},
  author={Gustavo Ferreira Martins and Bruno A. M. Guedes and Lucyane Mendes Silva and Jos{\'e} Eduardo Serr{\~a}o and Consuelo Latorre Fortes-Dias and Jos{\'e} Marcelo Ramalho-Ortig{\~a}o and Paulo Filemon Paolucci Pimenta},
  journal={Tissue & cell},
  year={2011},
  volume={43 2},
  pages={83-90}
}
Oenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells… CONTINUE READING

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