Isolation, physicochemical properties, and the macromolecular composition of the vitelline and fertilization envelopes from Xenopus laevis eggs.

@article{Wolf1976IsolationPP,
  title={Isolation, physicochemical properties, and the macromolecular composition of the vitelline and fertilization envelopes from Xenopus laevis eggs.},
  author={Don P Wolf and Tsutomu Nishihara and D. M. West and R E Wyrick and Jerry L. Hedrick},
  journal={Biochemistry},
  year={1976},
  volume={15 17},
  pages={3671-8}
}
As a step toward defining in molecular terms the sperm-triggered block to polyspermy reaction established by the egg at fertilization, vitelline (VE) and fertilization (FE) envelopes were isolated from eggs of the Sounth African clawed toad Xenopus laevis and some of their physicochemical properties determined. Envelopes were isolated after lysis of the fertilized or unfertilized eggs by sieving techniques; isolated envelopes retained their in situ morphology as determined by electron… CONTINUE READING