Isolation, physicochemical properties, and macromolecular composition of zona pellucida from porcine oocytes.

@article{Dunbar1980IsolationPP,
  title={Isolation, physicochemical properties, and macromolecular composition of zona pellucida from porcine oocytes.},
  author={Bonnie S. Dunbar and Nate J Wardrip and Jerry L. Hedrick},
  journal={Biochemistry},
  year={1980},
  volume={19 2},
  pages={356-65}
}
Oocytes released en masse from pig ovaries were isolated in large quantities by using sieving techniques. The isolated oocytes were gently homogenized, and the largely intact zona pellucida "ghosts" were purified by using sieving techniques. Sufficient amounts of zonae were recovered to permit, for the first time, adequate characterization of the zona pellucida in chemical, physical, and macromolecular terms. The isolated zonae were greater than 93% pure as determined by chemical, enzymatic… CONTINUE READING

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