Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene.

@article{Verkamp1989IsolationNS,
  title={Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene.},
  author={Elizabeth Verkamp and Barry K. Chelm},
  journal={Journal of bacteriology},
  year={1989},
  volume={171 9},
  pages={
          4728-35
        }
}
The Escherichia coli hemA gene, essential for the synthesis of 5-aminolevulinic acid (ALA), was isolated and sequenced. The following criteria identified the cloned gene as hemA. (i) The gene complemented a hemA mutation of E. coli. (ii) The gene was localized to approximately 26.7 min on the E. coli chromosomal linkage map, consistent with the location of the mapped hemA locus. Furthermore, DNA sequence analysis established that the cloned gene lay directly upstream of prfA, which encodes… CONTINUE READING

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