• Corpus ID: 15637859

Isolation, nmr spectral assignments and partial synthesis of rebaudioside b; a sweet component of stevia rebaudiana

@inproceedings{Chaturvedula2011IsolationNS,
  title={Isolation, nmr spectral assignments and partial synthesis of rebaudioside b; a sweet component of stevia rebaudiana},
  author={Venkata Sai Prakash Chaturvedula and Indra Prakash},
  year={2011}
}

• V. S. Chaturvedula, I. Prakash
• Published 1 October 2011
• Chemistry

From the commercial extract of the leaves of Stevia rebaudiana, a diterpene glycoside was isolated which was characterized as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid (1); also known as rebaudioside B. The complete 1H and 13C NMR assignment of rebaudioside B was achieved by the extensive NMR (1H and 13C, COSY, HSQC, HMBC) and mass spectral data. Hydrolysis studies were performed on rebaudioside B using various acid, and enzymatic studies to identify aglycone and sugar residues in its structure. Further, partial synthesis of rebaudioside B was achieved from the alkaline hydrolysis of rebaudiosides A and D.