Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

  title={Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells},
  author={Simone Bonato Luisi and Manoel Sant’Ana Filho and Patricia Pranke},
Objective: The aim of this study has been the isolation and characterization of stem cells from dental pulp (DPSCs) in culture. Methods: The primary DPSCs cultures were obtained from human third molars. Immediately after extraction, the teeth were placed in Dulbecco’s modified Eagle medium (DMEM) culture medium supplemented with fetal bovine serum and antibiotics. In a laminar flow, the pulp was removed from the tooth, incubated with collagenase for 2 hours and placed on a culture plate… 

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