Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

  title={Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells},
  author={S. B. Luisi and Manoel Sant’Ana Filho and P. Pranke},
Objective: The aim of this study has been the isolation and characterization of stem cells from dental pulp (DPSCs) in culture. Methods: The primary DPSCs cultures were obtained from human third molars. Immediately after extraction, the teeth were placed in Dulbecco’s modified Eagle medium (DMEM) culture medium supplemented with fetal bovine serum and antibiotics. In a laminar flow, the pulp was removed from the tooth, incubated with collagenase for 2 hours and placed on a culture plate… CONTINUE READING
2 Citations

Figures from this paper


Isolation and characterization of postnatal stem cells from human dental tissues.
  • 343
Differentiation potential of STRO-1+ dental pulp stem cells changes during cell passaging
  • 155
A modified efficient method for dental pulp stem cell isolation
  • 15
Isolation of pluripotent stem cells from human third molar dental pulp.
  • 65
  • Highly Influential
  • PDF
Stem Cell Properties of Human Dental Pulp Stem Cells
  • 1,680
  • PDF
Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis
  • 111
  • Highly Influential
  • PDF
Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.
  • 3,443
  • PDF