Isolation, identification and characterization of Bacillus subtilis ZJB-063, a versatile nitrile-converting bacterium

@article{Zheng2007IsolationIA,
  title={Isolation, identification and characterization of Bacillus subtilis ZJB-063, a versatile nitrile-converting bacterium},
  author={Yuguo Zheng and Jing Chen and Zhi-Qiang Liu and Ming-Huo Wu and Liang-Ying Xing and Yin-Chu Shen},
  journal={Applied Microbiology and Biotechnology},
  year={2007},
  volume={77},
  pages={985-993}
}
Strain ZJB-063, a versatile nitrile-amide-degrading strain, was newly isolated from soil in this study. Based on morphology, physiological tests, Biolog and the 16S rDNA sequence, strain ZJB-063 was identified as Bacillus subtilis. ZJB-063 exhibited nitrilase activity without addition of inducers, indicating that the nitrilase in B. subtilis ZJB-063 is constitutive. Interestingly, the strain exhibited nitrile hydratase and amidase activity with the addition of ɛ-caprolactam. Moreover, the… CONTINUE READING