Isolation, growth requirements, cloning, prostacyclin production and life-span of human adult endothelial cells in low serum culture medium

  title={Isolation, growth requirements, cloning, prostacyclin production and life-span of human adult endothelial cells in low serum culture medium},
  author={Hiroyoshi Hoshi and Wallace L. Mckeehan},
  journal={In Vitro Cellular \& Developmental Biology},
SummaryEndothelial cells from autopsy and biopsy specimens from a variety of adult human vascular tissue were harvested by collagenase treatment and gentle swabbing of the lumenal surface. Nutrient medium MCDB 107 containing a partially purified brain-derived growth factor (5 μg/ml), epidermal growth factor (10 ng/ml) and only 2% (v/v) fetal bovine serum supported clonal and long-term serial culture (17.6 to 26.1 cumulative population doublings) of endothelial cells from vena cava, thoracic… 
Heparin-binding growth factor type one and platelet-derived growth factor are required for the optimal expression of cell surface low density lipoprotein receptor binding activity in human adult arterial smooth muscle cells
The results suggest that HBGF-1, PDGF, and LDL act together to promote the maximal proliferation of smooth muscle cells in culture and may contribute to the smooth muscle cell hyperplasia and lipid accumulation observed in atherosclerotic lesions.
Different hormonal requirements for androgen-independent growth of normal and tumor epithelial cells from rat prostate
The results suggest that anti-hormonal therapies against prostate tumor growth should be based on intervention with the activity of insulin (or insulin-like factors) or simultaneous intervention with both EGF and prostatropin (or their homologues).
Coordinated regulation of vascular endothelial cell growth and prostacyclin production by epidermal growth factor: Evidence of clonal variations among rat aortic endothelial cells
Rat aortic endothelial cells were found to exhibit clonal variations in response to EGF stimulation in cell growth and prostacyclin synthesis, implying a possible involvement of prostacyClin synthesis in some of the biological effects of EGF on vascular endothelial Cells.
Opposite effects of monokines (interleukin-1 and tumor necrosis factor) on proliferation and heparin-binding (fibroblast) growth factor binding to human aortic endothelial and smooth muscle cells
The results suggest that the two monokines may depress endothelial cell regeneration and augment smooth muscle cell hyperplasia by differential modulation of the HBGF receptor in the two vascular cell types.
Myogenic Properties of Human Mesenchymal Stem Cells Derived from Three Different Sources
For the further development of MSC-based treatments of myopathies, AT-MSCs appear to be the best choice in view of their efficient contribution to myoregeneration, their high ex vivo expansion potential, and because their harvesting is less demanding than that of BM- or SM- MSCs.
Replicative Senescence is Present in Cardiac Microvascular Endothelial Cells
In the present study, using the in vitro culture system, it was found that increases in SA-beta-gal positive CMECs was associated with the increases of passage number of these cells, and the telomere length of CM ECs was shorter in older passage as compared with younger passage.
A relationship exists between replicative senescence and cardiovascular health
Results are consistent with the model in which the inherited replicative capacity of vascular cells is a stronger determinant of the onset of cardiovascular disease later in life, than wear-and-tear throughout the life course.
A comprehensive review on mesenchymal stem cell growth and senescence.
This review summarizes recent advances in the understanding of the factors affecting MSC expansion in vitro and discusses the pattern of their senescence with particular emphasis on the role of telomere shortening, activation of effectory pathways, and oxidative stress.


Long-term culture of human endothelial cells
Newborn bovine serum is superior to fetal bovines serum for the proliferation of human umbilical vein endothelial cells seeded at low density in the presence of the growth factor.
Cultured endothelial cells derived from the human iliac arteries
Although the cultures described in this report were derived from patients with varying degrees of atherosclerotic disease, there were no significant differences in morphological or physiological parameters among these cultures or in comparison with commonly studied cells derived from human umbilical veins.
Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture.
  • H. Hoshi, W. Mckeehan
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
The results provide a simplified model for elucidation of the endocrinology of human endothelial cell growth, function, and aging and suggest an endocrine role of both the nervous system and liver in the regeneration of endothelial cells.
Properties of Cultured Endothelium from Adult Human Vessel
Prostacyclln production stimulated by incubation with arachldonlc acid and PGH2 was variable from vessel to vessel, however, average values were lower than normally seen with cultured primary umbilical vein endothelium.
Factors which stimulate the growth of human umbilical vein endothelial cells in vitro
The inability to grow large numbers of HUV endothelial cells has made studies of a variety of cell properties quite difficult and has significantly hindered rapid elucidation of the cell biology of the human endothelium.
Human endothelial cells: use of heparin in cloning and long-term serial cultivation.
The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.
A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro.
Much of the growth-promoting activity of dialyzed serum is directly or indirectly derived from platelets, which has important implications for the response of arteries to localized injury and provides a key to further understanding of the role of factors derived from blood serum in promoting cell proliferation in vitro.
Calcium, magnesium, and serum factors in multiplication of normal and transformed human lung fibroblasts
A sequential effector relationship among serum factors, Ca2+ and Mg2+, in a proliferative control system for normal cells is proposed, and Alteration or bypass of an intracellular Mg 2+-requiring process is proposed as a major lesion in the transformed cells.