Isolation, growth requirements, cloning, prostacyclin production and life-span of human adult endothelial cells in low serum culture medium

@article{Hoshi2007IsolationGR,
  title={Isolation, growth requirements, cloning, prostacyclin production and life-span of human adult endothelial cells in low serum culture medium},
  author={Hiroyoshi Hoshi and Wallace L. Mckeehan},
  journal={In Vitro Cellular \& Developmental Biology},
  year={2007},
  volume={22},
  pages={51-56}
}
SummaryEndothelial cells from autopsy and biopsy specimens from a variety of adult human vascular tissue were harvested by collagenase treatment and gentle swabbing of the lumenal surface. Nutrient medium MCDB 107 containing a partially purified brain-derived growth factor (5 μg/ml), epidermal growth factor (10 ng/ml) and only 2% (v/v) fetal bovine serum supported clonal and long-term serial culture (17.6 to 26.1 cumulative population doublings) of endothelial cells from vena cava, thoracic… 
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