Isolation, functional characterization, and transcriptome of Mastomys ileal enterochromaffin cells.

@article{Kidd2006IsolationFC,
  title={Isolation, functional characterization, and transcriptome of Mastomys ileal enterochromaffin cells.},
  author={Mark S. Kidd and Irvin Mark Modlin and Geeta N Eick and Manich C. Champaneria},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  year={2006},
  volume={291 5},
  pages={
          G778-91
        }
}
Although the enterochromaffin (EC) cell is one of the primary neuroendocrine regulatory cells of the small intestine, the lack of a purified cell system has precluded characterization of the cell and limited precise physiological evaluation. We developed methodology to obtain a pure population of Mastomys ileal EC cells, evaluated their functional regulation, and defined the transcriptome. Mastomys ilea were everted, end ligated, pronase-collagenase digested, and Nycodenz gradient centrifuged… CONTINUE READING
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