Isolation, endocrine regulation and mRNA distribution of the 3‐hydroxy‐3‐methylglutaryl coenzyme A synthase (HMG‐S) gene from the pine engraver, Ips pini (Coleoptera: Scolytidae)

@article{Bearfield2006IsolationER,
  title={Isolation, endocrine regulation and mRNA distribution of the 3‐hydroxy‐3‐methylglutaryl coenzyme A synthase (HMG‐S) gene from the pine engraver, Ips pini (Coleoptera: Scolytidae)},
  author={Jeremy C. Bearfield and Christopher I. Keeling and Sharon Young and Gary J Blomquist and Claus Tittiger},
  journal={Insect Molecular Biology},
  year={2006},
  volume={15}
}
We isolated a full‐length cDNA encoding 3‐hydroxy‐3‐methylglutaryl coenzyme A synthase (HMG‐S) from the pine engraver beetle, Ips pini (Say), and examined its genomic structure. The intron‐less gene has a predicted 460 amino acid cytosolic protein product with 73% identity to HMG‐S from Dendroctonus jeffreyi, and high identity (58–64%) with other insect HMG‐Ss. Topically applied juvenile hormone (JH) III induced HMG‐S mRNA levels up to 6.5‐fold in both sexes, mostly in the anterior midgut… Expand
Isolation and expression of HMG-CoA synthase and HMG-CoA reductase genes in different development stages, tissues and treatments of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae).
TLDR
Increase of DaH MG-S and DaHMG-R mRNA levels in males suggested that they may play a role in mevalonate pathway, and information from the present study might contribute to understanding the relationship between D. armandi and its semiochemical production. Expand
Isolation , endocrine regulation and transcript distribution of a putative primary JH-responsive gene from the pine engraver , Ips pini ( Coleoptera : Scolytidae )
We isolated a cDNA of unknown function from a juvenile hormone III (JH III)-treated male midgut cDNA library prepared from the pine engraver beetle, Ips pini, and examined its genomic structure. TheExpand
Isolation, endocrine regulation and transcript distribution of a putative primary JH-responsive gene from the pine engraver, Ips pini (Coleoptera: Scolytidae).
TLDR
Ipi10G08 is likely a primary JH response gene and may provide insight into how this hormone exerts its actions, and immunoblot analysis indicated that although JH III highly induces transcript levels in females, protein levels are not similarly induced, whileprotein levels are induced in males. Expand
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It is demonstrated that HMGR is an essential enzyme in cantharidin biosynthesis in the blister beetle E. chinensis, which further verifies previous research results demonstrating that cantharaidin is synthesized de novo by the mevalonate pathway in blister beetles. Expand
Functional expression of a bark beetle cytochrome P450 that hydroxylates myrcene to ipsdienol.
TLDR
Quantitative real-time PCR experiments showed that feeding on host phloem induced CYP9T2 expression in males, but not females, and that basal expression levels are highest in male midguts, similar to other I. pini pheromone-biosynthetic genes. Expand
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The expression of mevalonate pathway genes in D. rhizophagus gut is not directed to the production of terpenoid pheromones, regardless of their frequent occurrence in the genus Dendroctonus. Expand
Effects of juvenile hormone on gene expression in the pheromone‐producing midgut of the pine engraver beetle, Ips pini
TLDR
This is the first microarray‐based study of the developmental and hormonal regulation of insect pheromone biosynthesis and sex differences in basal levels of mevalonate pathway genes were consistent with their role in male‐specific pheromsynthesis. Expand
Two Regulatory Mechanisms of Monoterpenoid Pheromone Production in Ips spp. Of Bark Beetles
TLDR
The data suggest that pheromone production in Ips is not uniformly controlled by JH III and feeding may stimulate the release of some other regulatory factor, perhaps a brain hormone, required for phermone production. Expand
Myrcene Hydroxylases do not Determine Enantiomeric Composition of Pheromonal Ipsdienol in Ips spp.
TLDR
Quantitative real-time PCR experiments showed that IcCYP9T1, as does CYP9T2, has an expression pattern similar to other pheromone-biosynthetic genes in I. pini, and results confirm further that Ips spp. Expand
Molecular characterization and expression of two enzymes from Dendroctonus armandi, with phloem feeding and juvenile hormone.
  • L. Dai, Yuanyuan Wang, H. Chen
  • Medicine, Biology
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2020
TLDR
Two genes encoding, (Z)-6-nonen-2-ol dehydrogenase and CYP6CR, that are known to be involved in xo-brevicomin synthesis are identified to improve the understanding of exo-Brevicomin biosynthesis in the Chinese white pine beetle. Expand
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