Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells

@article{Kuroda2013IsolationCA,
  title={Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells},
  author={Yasumasa Kuroda and Shohei Wakao and Masaaki Kitada and Toru Murakami and Makoto Nojima and Mari Dezawa},
  journal={Nature Protocols},
  year={2013},
  volume={8},
  pages={1391-1415}
}
Multilineage-differentiating stress-enduring (Muse) cells are distinct stem cells in mesenchymal cell populations with the capacity to self-renew, to differentiate into cells representative of all three germ layers from a single cell, and to repair damaged tissues by spontaneous differentiation into tissue-specific cells without forming teratomas. We describe step-by-step procedures for isolating and evaluating these cells. Muse cells are also a practical cell source for human induced… 

Muse Cells

  • M. Dezawa
  • Biology
    Advances in Experimental Medicine and Biology
  • 2018
Characteristics indicate that Muse cells correspond to the subpopulation of MSC cells responsible for the pluripotent aspect of M SCs, which play an important role in vivo as endogenous stem cells that contribute to tissue homeostasis through daily reparative maintenance and to tissue reconstruction through their unique reparatives functions following serious tissue damage.

In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells

The Muse Cell Discovery, Thanks to Wine and Science.

  • M. Dezawa
  • Biology
    Advances in experimental medicine and biology
  • 2018
Characteristics indicate that Muse cells correspond to the subpopulation of MSC cells responsible for the pluripotent aspect of M SCs, which play an important role in vivo as endogenous stem cells that contribute to tissue homeostasis through daily reparative maintenance and to tissue reconstruction through their unique reparatives functions following serious tissue damage.

Basic Characteristics of Muse Cells.

The properties exhibited by Muse cells are similar to those of primed pluripotent stem cells while with some uniqueness, and a comprehensive description of the basic characteristics of Muse cells is provided.

Muse Cells, a New Type of Pluripotent Stem Cell Derived from Human Fibroblasts.

This study provides an innovative and exciting platform for exploring the potential cell-based therapy of various human diseases using Muse cells as well as their great possibility for regenerative medicine.

Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue

Naïve pluripotent-like characteristics of non-tumorigenic Muse cells isolated from human amniotic membrane

HAMSC-SSEA-3(+) cells exhibited unique characteristics not seen in hBM-Muse cells, including higher expression of genes related to germline- and extraembryonic cell-lineages compared with those in hBMCs in single-cell RNA-sequencing, suggesting a broader differentiation potential similar to naïve pluripotent stem cells.

Neurotrophic Factor Secretion and Neural Differentiation Potential of Multilineage-differentiating Stress-enduring (Muse) Cells Derived from Mouse Adipose Tissue

Mouse Muse cells showed greater pluripotency-like characteristics, survival, neurotrophic factor secretion, and neuronal and glial-differentiation capacities than non-Muse cells, indicating that they may have better neural-regeneration potential.

Stress and stem cells: adult Muse cells tolerate extensive genotoxic stimuli better than mesenchymal stromal cells

The high ability of Muse cells to cope with genotoxic stress is related to their quick and efficient sensing of DNA damage and activation of DNA repair systems.
...

References

SHOWING 1-10 OF 49 REFERENCES

Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts

It is demonstrated that in adult human fibroblasts a subset of preexisting adult stem cells selectively become iPS cells, but the remaining cells make no contribution to the generation of i PS cells.

Unique multipotent cells in adult human mesenchymal cell populations

Adult human stem cells that can generate, from a single cell, cells with the characteristics of the three germ layers can be obtained through easily accessible adult human mesenchymal cells without introducing exogenous genes.

Multilineage differentiation potential of fibroblast-like stromal cells derived from human skin.

Cutting-edge isolation of progenitors from human foreskin tissue samples, which have fibroblast-like morphology and could be easily propagated for more than 50 passages, demonstrate that foreskin can serve as a valuable source of multipotent cells with the capabilities for endodermal, mesodmal, and ectodermal cells.

Multipotent mesenchymal stem cells from adult human synovial membrane.

It is demonstrated that human multipotent MSCs can be isolated from the SM of knee joints and have the ability to proliferate extensively in culture, and they maintain their multilineage differentiation potential in vitro, establishing their progenitor cell nature.

Mesenchymal cell populations: development of the induction systems for Schwann cells and neuronal cells and finding the unique stem cell population

  • M. Kitada
  • Biology
    Anatomical Science International
  • 2012
The stemness of MSCs is discussed and the discovery of a certain stem cell type among adult M SCs that can self-renew and differentiate into cells of all three germ layers are described.

Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy

It is shown that a tractable MPC population, human umbilical cord perivascular cells (HUCPVCs), was capable of multilineage differentiation in vitro and, more importantly, contributed to rapid connective tissue healing in vivo by producing bone, cartilage and fibrous stroma.

Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis.

It is demonstrated that, regardless of donor age, the adult human periosteum contains cells that, upon enzymatic release and culture expansion, are multipotent MSCs at the single-cell level.

Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro

The differentiation of expanded adult human MSCs into cells with phenotypic and functional features of endothelial cells are shown to provide new options for engineering of artificial tissues based on autologous M SCs and vascularized engineered tissues.

Multilineage potential of adult human mesenchymal stem cells.

Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.

Bone Marrow Mesenchymal Cells: How Do They Contribute to Tissue Repair and Are They Really Stem Cells?

Recent evidence suggests that a very small subpopulation of cells that assume a repair function with the ability to differentiate into trilineage cells resides among human MSCs and effective utilization of such cells is expected to improve the repair effect of MSCS.