Isolation, culture, and differentiation of echinoid primary mesenchyme cells

@article{Harkey1980IsolationCA,
  title={Isolation, culture, and differentiation of echinoid primary mesenchyme cells},
  author={M A Harkey and Arthur H. Whiteley},
  journal={Wilhelm Roux's archives of developmental biology},
  year={1980},
  volume={189},
  pages={111-122}
}
Methods are described for isolation and culture of primary mesenchyme cells from echinoid embryos. Ninety-five percentpure primary mesenchyme cells were isolated from early gastrulae ofStrongylocentrotus purpuratus, exploiting the biological segregation of these cells within the blastocoel. When cultured, more than 90% of the isolated cells reached the differentiated state, spicule formation, in synchrony with in vivo controls. Isolated primary mesenchyme cells were cultured with and without… CONTINUE READING

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