Isolation, chromosomal location, and expression analysis of putative powdery mildew resistance genes in wheat (Triticum aestivum L.)

@article{Wan2006IsolationCL,
  title={Isolation, chromosomal location, and expression analysis of putative powdery mildew resistance genes in wheat (Triticum aestivum L.)},
  author={P. N. Wan and Lijun Ling and Shuanghe Cao and Xianping Wang and Wenjun Zhang and Hongqing Ling and Lihuang Zhu and Xiangqi Zhang},
  journal={Euphytica},
  year={2006},
  volume={155},
  pages={125-133}
}
Special and degenerate primers are designed according to the conservative sequence of barley powdery mildew resistance genes Mla1, Mla6, and Mla13. Two wheat Mla-like orthologs, TaMla-2 and TaMla-3 are cloned and sequenced from the cDNA of wheat resistant-powdery mildew line TAM104R by RT-PCR method. TaMla-2 and TaMla-3 encode distinct but highly related coiled-coil nucleotide-binding site leucine-rich repeat type (NBS-LRR) resistant disease proteins and both reveal about 74 and 81% identity… CONTINUE READING

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TaMla-3 encode distinct but highly related coiled - coil nucleotide - binding site leucine - rich repeat type ( NBS - LRR ) resistant disease proteins and both reveal about 74 and 81% identity with amino acid sequence of Mla1 , respectively .
TaMla-3 encode distinct but highly related coiled - coil nucleotide - binding site leucine - rich repeat type ( NBS - LRR ) resistant disease proteins and both reveal about 74 and 81% identity with amino acid sequence of Mla1 , respectively .
TaMla-3 encode distinct but highly related coiled - coil nucleotide - binding site leucine - rich repeat type ( NBS - LRR ) resistant disease proteins and both reveal about 74 and 81% identity with amino acid sequence of Mla1 , respectively .
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