Isolation, characterization and localization of glycosaminoglycans in growing antlers of wapiti (Cervus elaphus).

@article{Sunwoo1998IsolationCA,
  title={Isolation, characterization and localization of glycosaminoglycans in growing antlers of wapiti (Cervus elaphus).},
  author={Hoon H. Sunwoo and Takuo Nakano and R. J. W. Hudson and Jeong Seop Sim},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={1998},
  volume={120 2},
  pages={273-83}
}
Glycosaminoglycans were isolated from the four sections (tip, upper, middle and base) of the main beam of growing antlers of wapiti (Cervus elaphus) by papain digestion and DEAE-Sephacel chromatography. Chondroitin sulfate was the major glycosaminoglycan in each section of antler accounting for, on average, 88% of the total uronic acid. The yield of chondroitin sulfate liberated from the tissue was approximately 6-fold greater in the cartilaginous (tip and upper) sections than in the bony… CONTINUE READING