Isolation, characterization, and inactivation of the APA1 gene encoding yeast diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase.

@article{Plateau1989IsolationCA,
  title={Isolation, characterization, and inactivation of the APA1 gene encoding yeast diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase.},
  author={Pierre Plateau and Michel Fromant and Jean Marie Schmitter and J. M. Buhler and Sylvain Blanquet},
  journal={Journal of bacteriology},
  year={1989},
  volume={171 12},
  pages={6437-45}
}
The gene encoding diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase from yeast was isolated from a lambda gt11 library. The DNA sequence of the coding region was determined, and more than 90% of the deduced amino acid sequence was confirmed by peptide sequencing. The Ap4A phosphorylase gene (APA1) is unique in the yeast genome. Disruption… CONTINUE READING