Isolation, characterization, and in vitro assembly of the tetragonally arrayed layer of Bacillus sphaericus.

@article{Hastie1979IsolationCA,
  title={Isolation, characterization, and in vitro assembly of the tetragonally arrayed layer of Bacillus sphaericus.},
  author={A T Hastie and Charles C. Brinton},
  journal={Journal of bacteriology},
  year={1979},
  volume={138 3},
  pages={999-1009}
}
The tetragonally arranged cell wall layer (T-layer) of Bacillus sphaericus NTCC 9602 was isolated and characterized. Parallel studies were made on a spontaneous variant of the wild-type strain which had a T-layer subunit of altered molecular weight. A purification method for the T-layers was devised which involved separation of the cell walls from the cytoplasmic contents, urea dissociation of the T-layer from the cell walls, removal of soluble contaminants by differential centrifugation, and… CONTINUE READING

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