Isolation, characterization, and genetic profiling of subpopulations of olfactory ensheathing cells from the olfactory bulb

@article{Honore2012IsolationCA,
  title={Isolation, characterization, and genetic profiling of subpopulations of olfactory ensheathing cells from the olfactory bulb},
  author={Axel Honoré and St{\'e}phanie S. Le Corre and C{\'e}line Derambure and Romain Normand and C{\'e}lia Duclos and Olivier Boyer and Jean Paul Marie and Nicolas Gu{\'e}rout},
  journal={Glia},
  year={2012},
  volume={60}
}
Olfactory ensheathing cells (OECs) play a crucial role during neurogenesis of primary olfactory neurons. Transplantation of OECs is considered as a promising new therapy for central nervous system repair. Nevertheless, OECs are constituted of distinct subpopulations and their role during neurogenesis is not clearly understood. In particular, OECs from the olfactory bulb (OB) constitute a heterogeneous, but not yet isolated and characterized, population of cells. In our study, flow cytometry… 
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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References

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TLDR
It appears that OB‐O ECs and OM‐OECs display distinct gene expression pattern, which suggest that they may be implicated in different physiological processes, and may represent a crucial point for future clinical application.
Comparison of bulbar and mucosal olfactory ensheathing cells using FACS and simultaneous antigenic bivariate cell cycle analysis
TLDR
Overall, the numbers of OECs in the cultures were inversely correlated with the deposition of fibronectin (FN), and a novel four color simultaneous antigenic bivariate cell cycle analysis shows that proliferation of Oecs is time‐limited, and is unable to prevent an overall loss of O ECs with time.
Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay
TLDR
It is found that the distinct OEC subpopulations exhibited different migratory properties based on time-lapse imaging of single isolated cells, possibly due to their different cytoskeletal organizations, and the notion of OECs as a single cell type with malleable functional phenotypes is supported.
Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions
TLDR
It is demonstrated that OECs from the olfactory bulb are a heterogeneous population that use lamellipodial waves to regulate cell–cell recognition and that inhibition of lamellIPodial wave activity via Mek1 abolished the ability of the cells to distinguish between each other.
Olfactory ensheathing cell membrane properties are shaped by connectivity
TLDR
It is concluded that the diversity of OEC current profiles can be explained by differences in gap‐junction connectivity and implications for OEC influences on axon growth and excitability are discussed.
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TLDR
It is shown that OECs in fact originate from the neural crest and hence share a common developmental heritage with Schwann cells, which overturns the existing dogma on the developmental origin of O ECs.
Lamina Propria and Olfactory Bulb Ensheathing Cells Exhibit Differential Integration and Migration and Promote Differential Axon Sprouting in the Lesioned Spinal Cord
TLDR
LP and OB OECs exhibit intrinsic biological differences that, after transplantation into the lesioned CNS, result in differences in postlesion spinal cord neuropathology and anatomical and behavioral regeneration outcomes that also vary depending on direct versus rostrocaudal transplantation.
SPARC from Olfactory Ensheathing Cells Stimulates Schwann Cells to Promote Neurite Outgrowth and Enhances Spinal Cord Repair
TLDR
Data provide the first evidence for a role for SPARC in modulating different aspects of CNS repair and indicate that SPARC can change the activation state of endogenous Schwann cells, resulting in the promotion of outgrowth in vitro, and in vivo.
Olfactory ensheathing glia: properties and function
Olfactory ensheathing cells and Schwann cells differ in their in vitro interactions with astrocytes
TLDR
The hypothesis that OECs will integrate more extensively than Schwann cells in astrocytic environments and are therefore better candidates for transplant‐mediated repair of the damaged CNS is tested.
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