Isolation, bulk cultivation, and characterization of coronary microvascular pericytes: the second most frequent myocardial cell type in vitro.

@article{Nees2012IsolationBC,
  title={Isolation, bulk cultivation, and characterization of coronary microvascular pericytes: the second most frequent myocardial cell type in vitro.},
  author={Stephan Nees and Dominik Roger Weiss and Anton Senftl and Mar{\'i}a Elena Knott and Stefan F{\"o}rch and Maximilian Schnurr and Peter A. Weyrich and Gerd Juchem},
  journal={American journal of physiology. Heart and circulatory physiology},
  year={2012},
  volume={302 1},
  pages={
          H69-84
        }
}
Densely arranged pericytes engird the endothelial tube of all coronary microvessels. Since the experimental access to these abundant cells in situ is difficult, a prerequisite for broader investigation is the availability of sufficient numbers of fully differentiated pericytes in homogenous culture. To reach this goal, we applied strictly standardized cell isolation techniques, optimized culture methods and specific histological staining. Approximately 1,000-fold enriched pericytes were… CONTINUE READING

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