Isolation, Purification and Partial Characterisation of Urease from Seeds of Water Melon (Citrullus vulgaris)

@article{Prakash2012IsolationPA,
  title={Isolation, Purification and Partial Characterisation of Urease from Seeds of Water Melon (Citrullus vulgaris)},
  author={Om Prakash and Gunjan Bhushan},
  journal={Journal of Plant Biochemistry and Biotechnology},
  year={2012},
  volume={6},
  pages={45-47}
}
  • O. Prakash, G. Bhushan
  • Published 2012
  • Biology, Chemistry
  • Journal of Plant Biochemistry and Biotechnology
Urease from seeds of water melon was purified to apparent homogeniety upto a sp act of 3750 units/mg protein with 31% recovery. Enzyme showed single protein band on native PAGE by urease specific staining. The mol wt of the enzyme was 4,70,000 and the preparation was free from bound nucleotides (A280/A260=1.14). The enzyme exhibited maximum activity in 50 mM Tris-acetate buffer (pH 8.5). The Km for urease was 8 mM. The enzyme was not inhibited by 25 mM of EDTA in 50 mM Tris-acetate buffer (pH 8… 
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18 Citations

Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds
TLDR
Urease was purified from germinating Pisum Sativum L. seeds to provide an insight on the various aspects of the property of the enzyme.
Inhibition of Urease from Seeds of Water-melon (Citrullus vulgaris) by Heavy Metal Ions
TLDR
Urease from the seeds of water melon was found to be inhibited by heavy metal ions like copper, lead, nickel and cobalt, and the inhibition was largely irreversible, hence could not be reversed by dialysis.
Characterization of urease derived from Citrullus lanatus (watermelon) seeds to estimate total Kjeldahl nitrogen in human urine
ABSTRACT A urease extract prepared by decanting liquid from a suspension of finely ground Citrullus lanatus (watermelon) seeds was characterized and applied to dilute urine samples to demonstrate a…
Physico-Chemical Characterization of Watermelon Urease upon Immobilization in Alginate Beads
TLDR
Watermelon (Citrullus vulgaris) urease was immobilized in 3% alginate leading to 72% immobilization and the enzyme was significantly activated by ME and it exhibited two peaks of activation; one at lower concentration and another at higher concentration.
Marine urease with higher thermostability, pH and salinity tolerance from marine sponge-derived Penicillium steckii S4-4
TLDR
This marine Urease produced by Penicillium steckii S4-4 derived from marine sponge Siphonochalina sp.
Molecular Asymmetry in Urease of Water Melon (Citrullus vulgaris)
TLDR
The data suggest the existence of half-and-half distribution of sites which is a manifestation of a pre-exlstent site heterogeneity in the oligomeric protein molecule.
Kinetics of Thermal Inactivation and Molecular Asymmetry of Urease from Dehusked Pigeongea (Cajanus cajan L.) Seeds
TLDR
The data suggests the existence of site-site heterogeneity in oligomeric urease molecule from pigeonpea and the time course of thermal inactivation can be described by the following equation, consisting of two first order terms: At = Afast + Aslow.
Inhibition of Urease by Disulfiram, an FDA-Approved Thiol Reagent Used in Humans
TLDR
It is shown that disulfiram inhibits urease in Citrullus vulgaris (CVU), following a non-competitive mechanism, and found that other thiol reactive compounds (l-captopril and Bithionol) and quercetin inhibits CVU, suggesting that the three drugs bind to the same subsite.
Kinetics Analysis of Thermal Inactivation of Enzyme used in Biotechnology using Variation Iteration Method
TLDR
The information given in this theoretical investigation will assist in the kinetic analysis of the experimental results over handling rate constants and molar concentrations and can serve as a starting point for a better understanding of the relationship between the physical quantities of the problems.
Picard Iteration Method to Kinetic Analysis of Thermal Inactivation of Enzyme as Applied in Biotechnology
In this work, Picard iteration method is used to obtain analytical expressions for the prediction of molar concentration of native and denatured jack bean urease (EC 3.5.1.5) through the…
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