Isolation, Purification and Partial Characterisation of Urease from Seeds of Water Melon (Citrullus vulgaris)

@article{Prakash2012IsolationPA,
  title={Isolation, Purification and Partial Characterisation of Urease from Seeds of Water Melon (Citrullus vulgaris)},
  author={Om Prakash and Gunjan Bhushan},
  journal={Journal of Plant Biochemistry and Biotechnology},
  year={2012},
  volume={6},
  pages={45-47}
}
  • O. Prakash, G. Bhushan
  • Published 2012
  • Biology, Chemistry
  • Journal of Plant Biochemistry and Biotechnology
Urease from seeds of water melon was purified to apparent homogeniety upto a sp act of 3750 units/mg protein with 31% recovery. Enzyme showed single protein band on native PAGE by urease specific staining. The mol wt of the enzyme was 4,70,000 and the preparation was free from bound nucleotides (A280/A260=1.14). The enzyme exhibited maximum activity in 50 mM Tris-acetate buffer (pH 8.5). The Km for urease was 8 mM. The enzyme was not inhibited by 25 mM of EDTA in 50 mM Tris-acetate buffer (pH 8… 
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References

SHOWING 1-10 OF 15 REFERENCES
Purification and properties of urease from bovine rumen.
TLDR
Polyacrylamide-gel electrophoresis showed that the mixed rumen bacteria produce ureases which have identical molecular weights and electrophoretic mobility.
Some properties and purifications of urease.
  • K. R. Lynn
  • Biology, Chemistry
    Biochimica et biophysica acta
  • 1967
Comparisons of soybean urease isolated from seed and tissue culture.
Dissociation of Urease by Glycol and Glycerol
TLDR
It is shown that highly purified preparations of jack bean urease can be reversibly and rapidly dissociated without appreciable change in enzyme activity.
On the substrate specificity of jack bean urease (urea amidohydrolase, EC 3.5.1.5).
pH-Stat studies prove that N-methylurea, ethyl carbamate, and carbamoyl azide are not substrates for jack bean urease. Semicarbazide is a good substrate, showing Michaelis–Menten kinetics and stoic...
THE ISOLATION AND CRYSTALLIZATION OF THE ENZYME UREASE PRELIMINARY PAPER
TLDR
A means of obtaining from the jack bean a new protein which crystallizes beautifully and whose solutions possess to an extraordinary degree the ability to decompose urea into ammonium carbonate is discovered.
...
...