Isolation, Purification and Partial Characterisation of Urease from Seeds of Water Melon (Citrullus vulgaris)

  title={Isolation, Purification and Partial Characterisation of Urease from Seeds of Water Melon (Citrullus vulgaris)},
  author={Om Prakash and Gunjan Bhushan},
  journal={Journal of Plant Biochemistry and Biotechnology},
  • O. Prakash, G. Bhushan
  • Published 2012
  • Biology, Chemistry
  • Journal of Plant Biochemistry and Biotechnology
Urease from seeds of water melon was purified to apparent homogeniety upto a sp act of 3750 units/mg protein with 31% recovery. Enzyme showed single protein band on native PAGE by urease specific staining. The mol wt of the enzyme was 4,70,000 and the preparation was free from bound nucleotides (A280/A260=1.14). The enzyme exhibited maximum activity in 50 mM Tris-acetate buffer (pH 8.5). The Km for urease was 8 mM. The enzyme was not inhibited by 25 mM of EDTA in 50 mM Tris-acetate buffer (pH 8… 
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