Isolation, Propagation, Characterization, Cryopreservation, and Application of Novel Cardiovascular Endothelial Cell Line From Channa striatus (Bloch, 1793)

@article{Majeed2014IsolationPC,
  title={Isolation, Propagation, Characterization, Cryopreservation, and Application of Novel Cardiovascular Endothelial Cell Line From Channa striatus (Bloch, 1793)},
  author={Seepoo Abdul Majeed and Kalaiselvi S Nathiga Nambi and Gani Taju and Azeez Sait Sahul Hameed},
  journal={Cell Biochemistry and Biophysics},
  year={2014},
  volume={71},
  pages={601-616}
}
There are only few primary endothelial cell cultures developed from fishes to date, but in this work the development of an endothelial cell line from Channa striatus is described. The vascular explants were plated into fibronectin (5 µg ml−1) and anti-CD31 antibody (100 ng ml−1)-coated flask; after 60 h incubation explants were removed from the flask. The flask contained only endothelial and blood cells. Blood cells were cleared out after subsequent passages. The culture medium used was… CONTINUE READING