Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages.

@article{PinedaTorra2015IsolationCA,
  title={Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages.},
  author={I Pineda-Torra and Matthew C. Gage and Alba de Juan and Oscar M Pello},
  journal={Methods in molecular biology},
  year={2015},
  volume={1339},
  pages={101-9}
}
Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various pathophysiological settings. This chapter outlines protocols used to isolate and… CONTINUE READING
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