Isolation, Characterization, and Immunogenicity of Mycoplasma pneumoniae Membranes.

  title={Isolation, Characterization, and Immunogenicity of Mycoplasma pneumoniae Membranes.},
  author={J. Pollack and N. Somerson and L. Senterfit},
  journal={Infection and immunity},
  volume={2 3},
Membrane and soluble fractions of Mycoplasma pneumoniae, M. pulmonis, and M. laidlawii B were prepared by hypotonic lysis of whole cells. The membranes of M. pneumoniae and M. laidlawii B contained, as percentage of dry weight: 34 to 37% protein, 59 to 61% lipid, 3 to 4% carbohydrate as hexose, and 0.2% ribonucleic acid as ribose. NADH(2) and NADPH(2) oxidase activities were localized in the soluble fractions of M. pneumoniae and in the membrane fraction of M. laidlawii B. NADH(2) oxidase… Expand
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