Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis.

@article{Weinmann2002IsolatingHT,
  title={Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis.},
  author={Amy S. Weinmann and Pearlly Yan and Matthew J Oberley and Tim Hui-Ming Huang and Peggy J. Farnham},
  journal={Genes & development},
  year={2002},
  volume={16 2},
  pages={235-44}
}
Previously, identification of promoters regulated by mammalian transcription factors has relied upon overexpression studies. Here we present the identification of a large set of promoters that are bound by E2F in physiological conditions. Probing a human CpG microarray with chromatin immunoprecipitated using an antibody to E2F4, we have identified 68 unique target loci; 15% are bidirectional promoters and 25% recruit E2F via a mechanism distinct from the defined consensus site. Interestingly… CONTINUE READING
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