Isolating and engineering human antibodies using yeast surface display

  title={Isolating and engineering human antibodies using yeast surface display},
  author={Ginger Chao and Wai Leung Lau and Benjamin J. Hackel and Stephen L Sazinsky and Shaun M. Lippow and K Dane Wittrup},
  journal={Nature Protocols},
This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display. Single-chain antibody fragments (scFvs) are first isolated from an existing nonimmune human library displayed on the yeast surface using magnetic-activated cell sorting selection followed by selection using flow cytometry. This enriched population is then mutagenized, and successive rounds of random mutagenesis and flow cytometry selection are… CONTINUE READING
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