Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment.

  title={Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment.},
  author={T Kaeberlein and Kim Lewis and Slava S. Epstein},
  volume={296 5570},
The majority (>99%) of microorganisms from the environment resist cultivation in the laboratory. Ribosomal RNA analysis suggests that uncultivated organisms are found in nearly every prokaryotic group, and several divisions have no known cultivable representatives. We designed a diffusion chamber that allowed the growth of previously uncultivated microorganisms in a simulated natural environment. Colonies of representative marine organisms were isolated in pure culture. These isolates did not… CONTINUE READING
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