Isolated wheat microspore culture

@article{Gustafson2004IsolatedWM,
  title={Isolated wheat microspore culture},
  author={Vicki D. Gustafson and P. Stephen Baenziger and Martha S. Wright and Walter W. Stroup and Yang Yen},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={42},
  pages={207-213}
}
The use of doubled haploid plants in a wheat breeding program requires an efficient haploid production system. While the techniques for producing doubled haploids from anther culture are well established, those for isolated microspores are complicated and inefficient. Four methods of isolating microspores from anthers (blending, stirring, macerating, and floating) were compared. Isolated microspores were washed and cultured in liquid medium. The effects of pre-isolation mannitol conditioning… CONTINUE READING