Isolated tumor endothelial cells maintain specific character during long-term culture.

@article{Matsuda2010IsolatedTE,
  title={Isolated tumor endothelial cells maintain specific character during long-term culture.},
  author={Kohei Matsuda and Noritaka Ohga and Yasuhiro Hida and Chikara Muraki and Kunihiko Tsuchiya and Takuro Kurosu and Tomoshige Akino and Shou-Ching Shih and Yasunori Totsuka and Michael Klagsbrun and Masanobu Shindoh and Kyoko Hida},
  journal={Biochemical and biophysical research communications},
  year={2010},
  volume={394 4},
  pages={947-54}
}
Tumor angiogenesis is necessary for solid tumor progression and metastasis. Increasing evidence indicates that tumor endothelial cells (TECs) are more relevant to the study of tumor angiogenesis than normal endothelial cells (NECs) because their morphologies and gene expression are different from NECs. However, it is challenging to isolate and culture large numbers of pure ECs from tumor tissue since the percentage of ECs is only about 1-2% and tumor cells and fibroblasts easily overgrow them… CONTINUE READING