Corpus ID: 30017253

Isolated permeabilized rat heart cells as a model to study heart bioenergetics in situ.

@article{Kmmel1989IsolatedPR,
  title={Isolated permeabilized rat heart cells as a model to study heart bioenergetics in situ.},
  author={L. K{\"u}mmel},
  journal={Acta Universitatis Carolinae. Medica},
  year={1989},
  volume={35 1-2},
  pages={
          31-42
        }
}
  • L. Kümmel
  • Published 1989
  • Chemistry, Medicine
  • Acta Universitatis Carolinae. Medica
Isolated rat heart cells permeabilized by digitonin were examined as an experimental model to study heart bioenergetics. The cells displayed good parameters of oxidative phosphorylation (acceptor control ratio ca. 8 with pyruvate + malate). High ATPase activity detected in the cells was characterized. The ATPase activity was Ca-dependent (optimum [free Ca] ca. 400 nM), and Mg was necessary for its full activity. Double reciprocal plot l/v vs. 1/[free Ca] at physiological [free Ca] was linear… Expand
Strong Inference for Systems Biology
TLDR
A revised method of strong inference for systems biology is presented and applied to analyze longstanding questions in cardiac energy metabolism and shows how this logical framework combined with computational-based hypothesis testing illuminates unresolved questions regarding how the energetic state of the heart is maintained in response to changes in the rate of ATP hydrolysis. Expand