Isolated microspore culture of maize: effects of isolation technique, reduced temperature, and sucrose level

@article{Pescitelli1990IsolatedMC,
  title={Isolated microspore culture of maize: effects of isolation technique, reduced temperature, and sucrose level},
  author={S. M. Pescitelli and Clarence Dan Johnson and Joseph F. Petolino},
  journal={Plant Cell Reports},
  year={1990},
  volume={8},
  pages={628-631}
}
Improvements in ab initio microspore culture of maize are presented using a modified isolation technique, reduced temperature during early stages of culture, and an elevated sucrose level in the culture medium. Blending-isolation, using excised anthers, was less stressful on microspores than pressing anthers against a stainless steel sieve and resulted in a… CONTINUE READING

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