Isolated intestinal mucosa cells of high viability from guinea pig.

@article{Hegazy1983IsolatedIM,
  title={Isolated intestinal mucosa cells of high viability from guinea pig.},
  author={E. Hegazy and V H Lopez del Pino and M. Schwenk},
  journal={European journal of cell biology},
  year={1983},
  volume={30 1},
  pages={132-6}
}
A simple method for preparing isolated enterocytes from the guinea pig is described. The procedure allows isolation of cell suspensions with high yield, outstanding viability and free of mucus. The isolated enterocytes were structurally intact. They largely excluded Trypane blue (94%), and lost smaller amounts of LDH than preparations from others. The cells accumulated K+ (greater than 90 mM) during incubation at 37 degrees and transported uridine actively. Incorporation of uridine and… CONTINUE READING