Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants.

@article{Scharte2009IsoenzymeRO,
  title={Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants.},
  author={Judith Scharte and Hardy Sch{\"o}n and Zeina Tjaden and Engelbert Weis and Antje von Schaewen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2009},
  volume={106 19},
  pages={8061-6}
}
In source leaves of resistant tobacco, oxidative burst and subsequent formation of hypersensitive lesions after infection with Phytophthora nicotianae was prevented by inhibition of glucose-6-phosphate dehydrogenase (G6PDH) or NADPH oxidases. This observation indicated that plant defense could benefit from improved NADPH availability due to increased G6PDH activity in the cytosol. A plastidic isoform of the G6PDH-encoding gene, G6PD, displaying high NADPH tolerance was engineered for cytosolic… CONTINUE READING