Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications.

@article{Dard1992IsoenzymeAO,
  title={Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications.},
  author={M L Dard{\'e} and Bernard Bouteille and Madeleine Pestre-Alexandre},
  journal={The Journal of parasitology},
  year={1992},
  volume={78 5},
  pages={786-94}
}
Isoenzyme analysis was conducted on the tachyzoite stage of 35 Toxoplasma gondii isolates. Fifteen enzyme systems were studied after isoelectrofocusing of tachyzoite extracts in polyacrylamide or agarose gels. Six enzyme systems showed variable electrophoretic patterns: aspartate aminotransferase (EC 2.6.1.1), glutathione reductase (EC 1.6.4.2), glucose phosphate isomerase (EC 5.3.1.9), amylase (EC 3.2.1.1), acid phosphatase (EC 3.1.3.2), and propionyl esterase. Their combination allows the… CONTINUE READING