Isoelectric focussing of the catalytic subunit of (Na,K)-ATPase from pig kidney.

  title={Isoelectric focussing of the catalytic subunit of (Na,K)-ATPase from pig kidney.},
  author={Mutsuo Yamaguchi and Robert L. Post},
  journal={The Journal of biological chemistry},
  volume={258 8},
The catalytic subunit of sodium and potassium ion transport adenosine triphosphatase was isolated by sodium dodecyl sulfate-polyacrylamide electrophoresis and was subjected to isoelectric focussing on 3.5% acrylamide in 2% Triton X-100, 9 M urea, and 2% Bio-Lyte 3/10 from Bio-Rad Laboratories. At 20 degrees C this resolved 2 equal and closely spaced bands centered at pH 5.5 about 0.04 pH unit apart. The distribution of the polypeptide between the 2 bands came to a temperature-dependent… CONTINUE READING