Isobutanol production in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes

@article{Lee2012IsobutanolPI,
  title={Isobutanol production in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes},
  author={Won-Heong Lee and Seung-Oh Seo and Yi-Hyun Bae and Hong Nan and Yong-Su Jin and Jin-Ho Seo},
  journal={Bioprocess and Biosystems Engineering},
  year={2012},
  volume={35},
  pages={1467-1475}
}
Engineering of Saccharomyces cerevisiae to produce advanced biofuels such as isobutanol has received much attention because this yeast has a natural capacity to produce higher alcohols. In this study, construction of isobutanol production systems was attempted by overexpression of effective 2-keto acid decarboxylase (KDC) and combinatorial overexpression of valine biosynthetic enzymes in S. cerevisiae D452-2. Among the six putative KDC enzymes from various microorganisms, 2-ketoisovalerate… Expand
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