Is there a rational method to purify proteins? From expert systems to proteomics.

@article{Asenjo2004IsTA,
  title={Is there a rational method to purify proteins? From expert systems to proteomics.},
  author={Juan A. Asenjo and Barbara A. Andrews},
  journal={Journal of molecular recognition : JMR},
  year={2004},
  volume={17 3},
  pages={236-47}
}
The purification of recombinant proteins for therapeutic or analytical applications requires the use of several chromatographic steps in order to achieve a high level of purity. A range of techniques is available such as anion and cation exchange chromatography, which can be carried out at different pHs, and hence used at different steps, hydrophobic… CONTINUE READING