Is defect in phosphorylation of Nbs1 responsible for high radiosensitivity of T-lymphocyte leukemia cells MOLT-4?

@article{ezov2008IsDI,
  title={Is defect in phosphorylation of Nbs1 responsible for high radiosensitivity of T-lymphocyte leukemia cells MOLT-4?},
  author={Martina Řez{\'a}{\vc}ov{\'a} and Ale{\vs} Tich{\'y} and Jiřina V{\'a}vrov{\'a} and Doris Vokurkov{\'a} and Em{\'i}lie Luk{\'a}{\vs}ov{\'a}},
  journal={Leukemia research},
  year={2008},
  volume={32 8},
  pages={1259-67}
}
Mutations in NBS1 gene are related to higher occurrence of malignancies. In this work we studied response of T-lymphocyte leukemia cells MOLT-4 to ionizing radiation. We detected IRIF (ionizing radiation forming foci) containing histone gammaH2A.X, protein 53BP1, and Nbs1, which were formed around double-strand breaks of DNA. We found dose-dependent increase in foci number (colocalization of gammaH2A.X and 53BP1) and gammaH2A.X amount (integral optical density) 1h after irradiation. After the… CONTINUE READING