Is cyclic AMP dependent protein kinase responsible for the in vivo phosphorylation of tyrosine aminotransferase?

Undegraded tyrosine aminotransferase was purified to near homogeneity from rat liver and was confirmed to be a substrate for the beef heart cyclic AMP dependent protein kinase catalytic subunit. Specific antibody was used to quantitate the amount of phosphate incorporated into the enzyme. Phosphate incorporation was maximal at a catalytic subunit to… CONTINUE READING