The transition from learning on campus to elearning presents many challenges. One of the key challenges is the organisational culture, which may enhance or hinder e-learning implementation. In this chapter, we describe how the organisational culture shapes e-learning use at universities. We compare a School of Business and a School of Health Sciences. It is argued that strategies for e-learning have played a key role in shaping the organisational culture, which in turn shapes how e-learning is being used. The School of Business regarded efficient administration as the key driver while the School of Health Sciences regarded collaborative learning as the key driver for e-learning. We introduce the concepts of administrationcentered and learning-centered e-learning culture to pinpoint the difference identified. A challenge is to develop an e-learning culture that values both how e-learning can be used to enhance administration and learning.