Iron loading of cultured hepatocytes. Effect of iron on 5-aminolaevulinate synthase is independent of lipid peroxidation.

@article{Shedlofsky1983IronLO,
  title={Iron loading of cultured hepatocytes. Effect of iron on 5-aminolaevulinate synthase is independent of lipid peroxidation.},
  author={Steven I. Shedlofsky and Herbert L. Bonkowsky and Peter R. Sinclair and Jacqueline F. Sinclair and William J Bement and J S Pomeroy},
  journal={The Biochemical journal},
  year={1983},
  volume={212 2},
  pages={321-30}
}
Cultured chick embryo hepatocytes were iron-loaded with ferric nitrilotriacetate. Iron-loading was confirmed by both quantitative cellular iron determinations and ultrastructural studies. With iron-loading, lipid peroxidation, as detected by malonaldehyde released into the medium, occurred at a linear rate for 12h, after which time the rate of malonaldehyde production decreased. No cell toxicity, as detected by lactate dehydrogenase release, was noted. The amount of malonaldehyde recovered in… CONTINUE READING