Iron: its intracellular localization and possible role in cell division.


When HeLa cells are fixed with ethanol, extracted with 0.1 N HCl, and incinerated at 540 degrees C, all organic constituents and all ions studied are removed with the exception of iron. The gross outlines of cell structures are preserved and high concentrations of residual ash in interphase nucleoli and mitotic chromosomes suggest that there may be a shift in iron salts during the cell cycle. Experiments with cells isotopically labeled in proteins, nucleic acids, lipids, and polysaccharides indicate that the iron is bound to a polysaccharide. Addition of iron chelating agents to living cells causes a selective inhibition of DNA synthesis. These data suggest that iron may play a crucial role in the mitotic process.


Citations per Year

1,812 Citations

Semantic Scholar estimates that this publication has 1,812 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Robbins1970IronII, title={Iron: its intracellular localization and possible role in cell division.}, author={E . Robbins and Thoru Pederson}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1970}, volume={66 4}, pages={1244-51} }