Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording.

@article{Priel2007IonicRF,
  title={Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording.},
  author={Avi Priel and Ziv Gil and Vincent Moy and Karl L. Magleby and Shai D. Silberberg},
  journal={Biophysical journal},
  year={2007},
  volume={92 11},
  pages={
          3893-900
        }
}
Patch-clamp recording has revolutionized the study of ion channels, transporters, and the electrical activity of small cells. Vital to this method is formation of a tight seal between glass recording pipette and cell membrane. To better understand seal formation and improve practical application of this technique, we examine the effects of divalent ions, protons, ionic strength, and membrane proteins on adhesion of membrane to glass and on seal resistance using both patch-clamp recording and… CONTINUE READING

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Subtype-specific control of P2X receptor channel signaling by ATP and Mg2+.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2013
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